Regulatory

Part:BBa_K619891

Designed by: BYU IGEM team   Group: iGEM11_BYU_Provo   (2011-09-27)


Mutated prfA thermosensor from listeria

This is the prfA-UTR thermosensor from Listeria monocytogenes randomly mutated to produce a thermosensor which gives increased expression when switching from 30 to 35 degrees celsius. This part was created by TAQ-mediated random mutagenesis of part BBa_K619889

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None